Peptide Reconstitution Calculator
Calculate the concentration when mixing lyophilized peptides with bacteriostatic water.
Amount in the vial (milligrams)
Bacteriostatic water to add
Concentration
—
mcg per ml
Per Insulin Unit
—
mcg per unit (100u syringe)
How to Use This Calculator
- Enter the peptide amount – This is the total milligrams (mg) in your peptide vial. Common sizes include 5mg, 10mg, and 15mg.
- Enter the BAC water amount – This is how much bacteriostatic water you'll add. More water = lower concentration (easier to measure small doses). Less water = higher concentration.
- Read your results – The calculator shows your concentration in mcg/ml and how many micrograms are in each unit on a 100-unit insulin syringe.
Understanding the Math
The reconstitution formula is straightforward:
Concentration (mcg/ml) = (Peptide mg × 1000) ÷ Water ml
For insulin syringe units (100 units = 1 ml):
mcg per unit = Concentration ÷ 100
Common Reconstitution Examples
| Peptide | Water | Concentration | mcg/unit |
|---|---|---|---|
| 5mg | 2ml | 2,500 mcg/ml | 25 mcg |
| 5mg | 2.5ml | 2,000 mcg/ml | 20 mcg |
| 10mg | 2ml | 5,000 mcg/ml | 50 mcg |
| 10mg | 5ml | 2,000 mcg/ml | 20 mcg |
Frequently Asked Questions
There's no single "correct" amount. Using more water creates a lower concentration, making it easier to measure smaller doses accurately. A common approach is 1-2ml per 5mg of peptide. For very small doses, consider using more water.
1 milligram (mg) = 1,000 micrograms (mcg). Peptide vials are typically measured in mg, while doses are often measured in mcg. This calculator converts between the two automatically.
Most people use U-100 insulin syringes, which have 100 units per 1ml. This calculator assumes you're using a 100-unit syringe. The "mcg per unit" result tells you how much peptide is in each unit marking on your syringe.
When stored properly in a refrigerator (2-8°C), most reconstituted peptides remain stable for 4-6 weeks. The bacteriostatic water contains benzyl alcohol which helps prevent bacterial growth. Never freeze reconstituted peptides.