Dilution Calculator
Calculate how to dilute a concentrated solution to your desired strength.
mcg/ml (stock solution)
mcg/ml (target)
ml total
Stock Solution Needed
—
ml of concentrated solution
Diluent to Add
—
ml of BAC water or saline
How to Use This Calculator
- Enter your current concentration – This is the strength of your existing solution in mcg/ml. You can get this from the reconstitution calculator or your mixing notes.
- Enter your desired concentration – This is how strong you want the final solution to be. A lower concentration means easier measurement of small doses.
- Enter the final volume you need – How much total diluted solution do you want to make?
- Mix according to results – Take the amount of stock solution shown and add the diluent amount. The total will equal your final volume at the desired concentration.
The Dilution Formula
This calculator uses the standard dilution equation:
C₁V₁ = C₂V₂
Where C₁ is initial concentration, V₁ is volume of stock solution, C₂ is final concentration, and V₂ is final total volume.
When to Use Dilution
- Easier measurement: Diluting a highly concentrated solution makes it easier to measure small doses accurately.
- Standardization: Create a consistent concentration across multiple vials.
- Reduce waste: Work with larger volumes to minimize the amount stuck in syringes and vials.
Frequently Asked Questions
Dilute when you've already reconstituted a peptide and find the concentration too high for accurate measurement, or when you want to create a standardized concentration across multiple vials. It's also useful if you need to measure very small doses that would be difficult with the original concentration.
Use the same type of solution you originally used for reconstitution, typically bacteriostatic water (BAC water). Using the same diluent maintains consistency and the preservative properties.
Diluting with bacteriostatic water generally maintains stability since the preservative (benzyl alcohol) is still present. However, a more dilute solution may have slightly reduced shelf life. Store refrigerated and use within the normal timeframe.